FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_9.3510000004686d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_9.3510000004686d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13560
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC350625.855457227138643TruSeq Adapter, Index 11 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT3162.3303834808259585TruSeq Adapter, Index 11 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT1240.9144542772861357TruSeq Adapter, Index 11 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCTTC1170.8628318584070797TruSeq Adapter, Index 11 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG930.6858407079646017RNA PCR Primer, Index 11 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCTT320.2359882005899705RNA PCR Primer, Index 11 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4450.040.4494445
ATGCCGT4950.036.3636444
CGTCTTC250.002034629236.045
GCTGCAT5150.035.38834832
CTGCATC5150.035.38834833
TCTCGTA5100.035.29411738
GCATCTC5100.035.29411735
GAGACAC5250.035.14285724
AGCCCAC5200.035.04807717
ACACGGC5200.035.04807727
TGCATCT5200.035.04807734
GCCCACG5200.035.04807718
CCACGAG5200.035.04807720
CACGAGA5200.035.04807721
GGCTGCA5200.035.04807731
CCCACGA5200.035.04807719
GACACGG5200.035.04807726
ACGAGAC5200.035.04807722
CGAGCCC5200.035.04807715
CCGAGCC5200.035.04807714