Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0120_pe_9.3510000004686d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13560 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC | 3506 | 25.855457227138643 | TruSeq Adapter, Index 11 (95% over 24bp) |
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT | 316 | 2.3303834808259585 | TruSeq Adapter, Index 11 (95% over 23bp) |
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT | 124 | 0.9144542772861357 | TruSeq Adapter, Index 11 (96% over 25bp) |
ATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCTTC | 117 | 0.8628318584070797 | TruSeq Adapter, Index 11 (96% over 27bp) |
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG | 93 | 0.6858407079646017 | RNA PCR Primer, Index 11 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCTT | 32 | 0.2359882005899705 | RNA PCR Primer, Index 11 (96% over 26bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 445 | 0.0 | 40.44944 | 45 |
ATGCCGT | 495 | 0.0 | 36.36364 | 44 |
CGTCTTC | 25 | 0.0020346292 | 36.0 | 45 |
GCTGCAT | 515 | 0.0 | 35.388348 | 32 |
CTGCATC | 515 | 0.0 | 35.388348 | 33 |
TCTCGTA | 510 | 0.0 | 35.294117 | 38 |
GCATCTC | 510 | 0.0 | 35.294117 | 35 |
GAGACAC | 525 | 0.0 | 35.142857 | 24 |
AGCCCAC | 520 | 0.0 | 35.048077 | 17 |
ACACGGC | 520 | 0.0 | 35.048077 | 27 |
TGCATCT | 520 | 0.0 | 35.048077 | 34 |
GCCCACG | 520 | 0.0 | 35.048077 | 18 |
CCACGAG | 520 | 0.0 | 35.048077 | 20 |
CACGAGA | 520 | 0.0 | 35.048077 | 21 |
GGCTGCA | 520 | 0.0 | 35.048077 | 31 |
CCCACGA | 520 | 0.0 | 35.048077 | 19 |
GACACGG | 520 | 0.0 | 35.048077 | 26 |
ACGAGAC | 520 | 0.0 | 35.048077 | 22 |
CGAGCCC | 520 | 0.0 | 35.048077 | 15 |
CCGAGCC | 520 | 0.0 | 35.048077 | 14 |