FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_89.35100000047254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_89.35100000047254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6400
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGTC156724.484375No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCGT2353.671875No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAGGTTCATCTCGTATGCCG1532.390625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1900.043.8157945
ATGCCGT2200.037.84090844
AGCCCAC2500.033.317
GTATGCC2500.033.342
GCCCACG2500.033.318
CCACGAG2500.033.320
TATGCCG2500.033.343
CATCTCG2500.033.336
GAGCCCA2500.033.316
GTTCATC2500.033.333
CACGAGA2500.033.321
GGTTCAT2500.033.332
CCCACGA2500.033.319
CTAGGTT2500.033.329
ACGAGAC2500.033.322
CGTATGC2500.033.341
CGAGCCC2500.033.315
CCGAGCC2500.033.314
AGGTTCA2500.033.331
TCGTATG2500.033.340