FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_88.3510000004722d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_88.3510000004722d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC650.9428488540760082No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT180.26109660574412535No Hit
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA180.26109660574412535No Hit
GTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGTGTAACGGA170.24659123875834058No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC140.20307513780098638No Hit
TAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC130.18856977081520163No Hit
GGTTTGTTTGAGGTTCTAACCTTGGCCCGTGAATCCGGGTCGGGGACAGTG120.17406440382941687No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGAT120.17406440382941687No Hit
ACCATGCACTGTCCCCGACCCGGATTCACGGGCCAAGGTTAGAACCTCAAA110.15955903684363215No Hit
GTCGGACATCGTACCTAAAGTGCAATGGCAAAAGCGTGCTTAACTGCGAGA110.15955903684363215No Hit
GTTCGAAGGTACGCTTGGTACGGTCGGACATCGTACCTAAAGTGCAATGGC90.13054830287206268No Hit
GGACATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATA90.13054830287206268No Hit
GTGTAACGGAGGAGTTCGAAGGTACGCTTGGTACGGTCGGACATCGTACCT80.11604293588627793No Hit
TAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC80.11604293588627793No Hit
ATGTCCGACCGTACCAAGCGTACCTTCGAACTCCTCCGTTACACTTTGGGA70.10153756890049319No Hit
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAA70.10153756890049319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTC250.001964555636.0000048
Produced by FastQC (version 0.11.2)