FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_85.351000000471ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_85.351000000471ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55229
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC617411.178909630809901No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT9761.7671875282912963No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT3530.639156964638143No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG880.15933658041970702No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.12855565011135453No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA700.12674500715203968No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.12312372123340998No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12131307827409513No Hit
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC660.11950243531478028TruSeq Adapter, Index 9 (95% over 22bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA640.11588114939615056No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC620.11225986347752087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA590.10682793459957632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8100.039.44444745
TCTAATT250.002089815736.02
GCCGTCT1000.036.045
ATGCCGT8950.035.69832644
CGAGACC9350.034.65240523
GATCTCG9050.034.55801436
GACCGAG9400.034.46808626
CGAGTTA9400.034.46808629
AGATCTC9150.034.4262335
ACCGAGT9350.034.41176227
TATGCCG9300.034.3548443
CTCGTAT9250.034.297339
TAGATCT9250.034.297334
GAGTTAG9450.034.28571730
ACGAGAC9450.034.28571722
CCGAGTT9450.034.28571728
AGACCGA9450.034.28571725
GAGACCG9450.034.28571724
ATCTCGT9150.034.1803337
CCACGAG9550.034.16230420
Produced by FastQC (version 0.11.2)