FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_84.351000000471a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_84.351000000471a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46343
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC537711.602615281703816No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT5721.2342748635176835No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT3080.6646095418941372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.15104762315775846No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA610.13162721446604667No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.1251537449021427No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.12299592171417474No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12083809852620676No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA550.11868027533823877No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT470.10141768983449495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6250.041.76000245
CACGAGA7200.036.562521
CCACGAG7150.036.50349820
CGAGACC7150.036.50349823
ACGAGAC7250.036.31034522
CCCACGA7200.036.2519
ATGCCGT7200.036.2544
TTGTTAT250.00208633536.01
CTCATAC250.00208633536.03
GAGACCG7250.036.024
GCCCACG7400.035.8783818
GTATGCC7350.035.81632242
TATGCCG7350.035.81632243
GATCTCG7300.035.7534236
GTTAGAT7250.035.68965532
AGATCTC7250.035.68965535
ACCGAGT7200.035.62527
CGAGTTA7200.035.62529
CCGAGTT7200.035.62528
CGTATGC7400.035.57432641
Produced by FastQC (version 0.11.2)