FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_83.35100000047198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_83.35100000047198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201090
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC167868.347506091799692No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT16960.8434034511910091No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9090.4520364016112189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.39285891889203844No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6510.32373564075786965No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6290.31279526580138245No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6110.303844049927893No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.29887115222039884No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5430.2700283455169327No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4680.23273161271072657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.23024516385697946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4490.22328310706648766No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4320.21482918096374756No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT4320.21482918096374756No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG4060.20189964692426277No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3990.19841861852901685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3820.18996469242627678No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.18499179471878263No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3240.1611218857228107No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.13078720970709634No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.12830076085334927No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2490.12382515291660451No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2400.11934954497985976No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.10791188025262322No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTA206.997237E-445.028875
TGCCGTC19200.043.23764445
ACCGAGT20950.039.6259127
CGAGTTA21050.039.54453729
ATGCCGT20900.039.5054244
AGACCGA21100.039.45083625
CCGAGTT21050.039.4376628
GAGTTAG21050.039.4376630
ATCTCGT21000.039.317337
CGTATGC21000.039.317341
GACCGAG21200.039.26474426
CACGAGA21200.039.26474421
AGTTAGA21200.039.15862331
TATGCCG21150.039.1448343
GTATGCC21150.039.1448342
GATCTCG21050.039.11702736
ACGAGAC21300.039.08040222
CCACGAG21250.039.06648620
GTTAGAT21150.039.03845632
CTCGTAT21100.039.02433839
Produced by FastQC (version 0.11.2)