H23LNBCXX_l01n01_bir_0120_pe_82.3510000004717e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_82.3510000004717e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79937
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC	5732	7.1706468844214815	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT	614	0.7681048825950436	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	266	0.33276205011446514	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT	241	0.3014874213443086	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	241	0.3014874213443086	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	227	0.283973629233021	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	213	0.2664598371217334	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	185	0.2314322528991581	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	180	0.2251773271451268	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	172	0.21516944593867668	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	162	0.2026595944306141	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	155	0.1939026983749703	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.19140072807335778	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	138	0.17263595081126387	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115	0.14386329234271988	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113	0.14136132204110738	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	104	0.13010245568385104	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.12885147053304477	No Hit
GATAGAAAGTGTTGCTTGTACTGATAGAAAGACAATCAGGAAAAGAAAGAA	100	0.125098515080626	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	99	0.12384752992981973	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.1188435893265947	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	94	0.11759260417578843	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG	86	0.10758472296933834	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	83	0.10383176751691957	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.9939037E-4	45.000004	33
CAGCTAT	20	6.9939037E-4	45.000004	14
GCAGCTA	20	6.9939037E-4	45.000004	13
TGCCGTC	685	0.0	42.372265	45
ATGCCGT	740	0.0	39.222977	44
TATGCCG	755	0.0	38.44371	43
GTATGCC	755	0.0	38.44371	42
CACGAGA	770	0.0	38.27922	21
GAGACCG	770	0.0	38.27922	24
CGAGACC	770	0.0	38.27922	23
ACGAGAC	770	0.0	38.27922	22
AGACCGA	765	0.0	38.235294	25
GACCGAG	760	0.0	38.190792	26
CGAGTTA	755	0.0	38.145695	29
CCACGAG	775	0.0	38.032257	20
GATCTCG	730	0.0	37.91096	36
ACCGAGT	760	0.0	37.894737	27
GTTAGAT	755	0.0	37.847683	32
TTAGATC	745	0.0	37.75168	33
ATCTCGT	735	0.0	37.653065	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content