FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_81.35100000047155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_81.35100000047155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24871
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC728529.29114229423827No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT6532.625547826786217No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT2130.8564191226729927No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG1750.703630734590487No Hit
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC830.3337220055486309TruSeq Adapter, Index 9 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTT380.15278838808250572RNA PCR Primer, Index 9 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7700.041.49350745
ATGCCGT8700.036.98275844
CGTATGC9250.035.02702741
CTCGTAT9250.035.02702739
GTATGCC9200.034.97282442
TCTCGTA9200.034.97282438
GATCTCG9150.034.91803436
GAGCCCA9300.034.8387116
TCGTATG9300.034.8387140
ATCTCGT9250.034.78378337
TATGCCG9250.034.78378343
CCACGAG9200.034.7282620
CACGAGA9200.034.7282621
GAGTTAG9200.034.7282630
ACGAGAC9200.034.7282622
TAGATCT9200.034.7282634
GAGACCG9200.034.7282624
CGAGACC9150.034.6721323
CCGAGCC9350.034.65240514
AGCCCAC9300.034.59677517