Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_0120_pe_81.35100000047155.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24871 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC | 7285 | 29.29114229423827 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGT | 653 | 2.625547826786217 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCT | 213 | 0.8564191226729927 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCG | 175 | 0.703630734590487 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTTC | 83 | 0.3337220055486309 | TruSeq Adapter, Index 9 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTCTT | 38 | 0.15278838808250572 | RNA PCR Primer, Index 9 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 770 | 0.0 | 41.493507 | 45 |
ATGCCGT | 870 | 0.0 | 36.982758 | 44 |
CGTATGC | 925 | 0.0 | 35.027027 | 41 |
CTCGTAT | 925 | 0.0 | 35.027027 | 39 |
GTATGCC | 920 | 0.0 | 34.972824 | 42 |
TCTCGTA | 920 | 0.0 | 34.972824 | 38 |
GATCTCG | 915 | 0.0 | 34.918034 | 36 |
GAGCCCA | 930 | 0.0 | 34.83871 | 16 |
TCGTATG | 930 | 0.0 | 34.83871 | 40 |
ATCTCGT | 925 | 0.0 | 34.783783 | 37 |
TATGCCG | 925 | 0.0 | 34.783783 | 43 |
CCACGAG | 920 | 0.0 | 34.72826 | 20 |
CACGAGA | 920 | 0.0 | 34.72826 | 21 |
GAGTTAG | 920 | 0.0 | 34.72826 | 30 |
ACGAGAC | 920 | 0.0 | 34.72826 | 22 |
TAGATCT | 920 | 0.0 | 34.72826 | 34 |
GAGACCG | 920 | 0.0 | 34.72826 | 24 |
CGAGACC | 915 | 0.0 | 34.67213 | 23 |
CCGAGCC | 935 | 0.0 | 34.652405 | 14 |
AGCCCAC | 930 | 0.0 | 34.596775 | 17 |