FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_80.3510000004713b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_80.3510000004713b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28123
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC16695.934644241368275RNA PCR Primer, Index 45 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT13044.636774170607687No Hit
ATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTTC4151.4756604914127227RNA PCR Primer, Index 45 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTT2360.8391707854780784RNA PCR Primer, Index 45 (95% over 23bp)
ATCCTACACAGATCGGTTCAAAGTCCAATGCAAAGCTACAGTAAAGGTTCA500.17779042065213527No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAGCGTGGACGCTAGTTCACGTGGAGC450.16001137858692174No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT410.14578814493475092No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.13156491128258008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1244532944564947No Hit
CTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT330.11734167763040927No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11378586921736657No Hit
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACGT300.10667425239128117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC451.8158971E-840.045
GCCGTCT2150.029.30232645
CTTATAC3500.028.9285721
CCGTCTT802.8073482E-622.50000244
TGCCGTC4400.022.545
TTATACA4550.022.2527472
ATGCCGT4550.021.75824244
GTATGCC4600.021.5217442
TATGCCG4600.021.5217443
ATCTCCG4500.021.510
CTTCCTT4400.021.47727229
ACCTTCC4200.021.42857227
AGACCTT4200.021.42857225
GACCTTC4200.021.42857226
TATACAC4850.021.3402063
CACATCT4650.021.2903237
GAGCCCA4650.021.29032316
GAGACCT4150.021.14457924
ACATCTC4700.021.063838
CGTATGC4700.021.0638341
Produced by FastQC (version 0.11.2)