H23LNBCXX_l01n01_bir_0120_pe_8.35100000046844.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_8.35100000046844.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28656
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTC	2414	8.424064768285874	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	155	0.5408989391401452	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	155	0.5408989391401452	No Hit
TTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTCT	141	0.49204355108877723	No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	138	0.48157453936348404	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	130	0.4536571747627024	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125	0.4362088218872138	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124	0.43271915131211613	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	103	0.3594360692350642	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98	0.3419877163595757	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGT	97	0.33849804578447795	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	81	0.2826633165829146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80	0.27917364600781686	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.265214963707426	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.21635957565605807	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	58	0.20240089335566724	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCC	51	0.17797319932998323	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	50	0.17448352875488554	No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG	49	0.17099385817978785	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCG	46	0.1605248464544947	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG	45	0.157035175879397	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	42	0.14656616415410384	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.14656616415410384	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	0.14307649357900615	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	39	0.1360971524288107	No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC	39	0.1360971524288107	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA	39	0.1360971524288107	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	38	0.132607481853713	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.12213847012841987	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	34	0.11864879955332215	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	29	0.10120044667783362	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	220	41.93182	45
ATGCCGT	230	40.108692	44
TCTGGAT	250	38.7	32
CTGGATC	250	38.7	33
TGGATCT	250	38.7	34
GCTCTGG	250	38.7	30
GTATGCC	245	38.57143	42
ATCTCGT	245	38.57143	37
TATGCCG	245	38.57143	43
TCTCGTA	245	38.57143	38
CGTATGC	245	38.57143	41
TCGTATG	245	38.57143	40
CTCGTAT	245	38.57143	39
CTCTGGA	255	37.941174	31
GACAGCT	255	37.941174	26
CCACGAG	255	37.941174	20
GGATCTC	255	37.941174	35
CACGAGA	255	37.941174	21
ACGAGAC	255	37.941174	22
AGACAGC	255	37.941174	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content