FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_76.351000000470b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_76.351000000470b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240464
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC219059.109471688069732RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT55122.2922350123095345No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10550.4387351121165746No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8460.3518198150242864No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG8210.3414232483864528No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA8180.3401756603899128No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6980.2902721405283119No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA6470.26906314458713154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA5950.24743828598043782No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT5920.2461906979838978No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5870.2441113846563311No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA5320.22123893805309736No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4350.18090025949830327No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4320.17965267150176326No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4070.16925610486392975No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT3940.1638498902122563No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3610.15012642225031606No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3400.14139330627453592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG2980.12392707432297558No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT2930.12184776099540887No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2860.11893672233681549No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC2860.11893672233681549No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC2850.11852085967130216No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC2810.11685740900924879No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT2790.11602568367822211No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2760.11477809568168208No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2730.11353050768514204No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2610.10854015569898197No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT2590.1077084303679553No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC2440.10147049038525519No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTTC207.0133555E-445.0114426
CCAAGGC207.0133555E-445.011443
GAGACGT207.020548E-445.00208324
GAGCCCT207.020548E-445.00208324
TGTTCGT207.0277473E-444.9927213
CGAGACG253.8852857E-544.9927223
TGCCGTC22150.043.0630145
TAATATA250.00210117836.0091554
TCCAAGG250.00210117836.0091552
ATGCCGT27550.034.6223544
ATCTCGT28100.033.70451437
TATGCCG28300.033.625343
CATCTCG28250.033.60518336
TCGTATG28200.033.5052240
GTATGCC28450.033.44801742
CGTATGC28400.033.42769241
TCTCGTA28450.033.28986738
CTCGTAT28500.033.2314739
TCCTTCA28600.033.2077431
GCCGTCT950.033.15253445
Produced by FastQC (version 0.11.2)