FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_73.35100000047056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_73.35100000047056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134303
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC2081215.496303135447459RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGT30462.2680059268966444No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG6270.46685479847806827No Hit
TTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCT4680.3484657825960701No Hit
ATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTCTTC2130.15859660618154472RNA PCR Primer, Index 45 (95% over 24bp)
TTTTTTCGTTGTGATATTCATTTTAGCACTATCTGAAATACGATTCGTTGC2060.1533845111427146No Hit
GTATATGGATGAAAAGGTCTCCAGAGAAACATGTATCATAAAGCAAAGACA2040.1518953411316203No Hit
ATTCCACATTTCCTGTAGTTGATATGCAGGTTTTGTACAATATTGGGTTTC2020.15040617112052598No Hit
CTTGTGTATCTAAACATAGTCTTACACTTTAATCCTGCTTGAGTTCAGACT1940.1444494910761487No Hit
TATCAACACCTTCTATTTGTCCAACTTTTGGTGTCAGTTAATGATGACATA1860.13849281103177144No Hit
AAATGGAATGGAATGAAATGGAATGGAATGGTATTTCATTCCATTCCATTT1720.12806862095411123No Hit
TAAGAGATGTCATTTGAATCCTGAAATGTTACTTCCAGGTGCTTTCAGTTG1470.10945399581543226No Hit
GTATATAGGAGACACTTTGTAGACAATAGGATTGTTTAATCCTTCTTGGCT1450.10796482580433796No Hit
CTACACATTTTATTTTTGAGGCTATCATAAAAGGGACTGAGTTATTGATTT1440.10722024079879079No Hit
GAGTAACTACGCTGTTGTAGGACTTTACTTCTATCCTAATAGTGTTGTTGA1440.10722024079879079No Hit
GTATAATGGTTGGAAACCAGTTATAGTTCTTCAAGTTTGCAAAGTAAATCA1430.10647565579324364No Hit
TTTCAGAGTCAAGGATAAATGTGTCAGAACAGAGTATAGTATTGAGCTTCC1360.10126356075441352No Hit
TACTTGTCTCTTTTGAATAACGCTAGCTTTTTTAACTGCGTTGTTTATTGT1360.10126356075441352No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG351.1985139E-745.0130356
GGCAGTA207.013889E-444.99627728
CGAGTAC302.1523847E-644.99627740
GCGGTGT253.8742626E-544.9962779
TCTTCGT207.013889E-444.99627741
TAGCGGT302.1523847E-644.9962777
CCTACCA207.013889E-444.9962779
GCGTTGT351.2020246E-744.99627738
TCTAGGG207.013889E-444.99627714
CCATGAC207.013889E-444.99627744
GCCTGAT207.013889E-444.99627740
CGCTAGC351.2020246E-744.99627721
CATGTCG253.8742626E-544.99627734
ACGAGTA302.1523847E-644.99627739
TGAGCAG302.1523847E-644.99627723
CTAGGGA207.013889E-444.99627715
TGGTCCA253.8742626E-544.99627718
TGCCGTC23100.042.75620345
ACGCTAG403.430123E-739.37174220
GCTAGCT403.430123E-739.37174222
Produced by FastQC (version 0.11.2)