FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_72.3510000004703c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_72.3510000004703c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13548
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC13399.883377620312961No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT2071.5279007971656333RNA PCR Primer, Index 17 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT550.40596397992323596No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA440.3247711839385887No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.2583407144966047No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.23619722468260998No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.23619722468260998No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.22143489813994688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.2140537348686153No Hit
ATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCTTC280.20667257159728372Illumina Paired End PCR Primer 2 (95% over 22bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA240.1771479185119575No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.16238559196929436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1328609388839681No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1328609388839681No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG180.1328609388839681No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA170.12547977561263654No Hit
TATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCTT170.12547977561263654Illumina Paired End PCR Primer 2 (95% over 21bp)
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA140.10333628579864186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1800.036.2544
TAGCGAT1950.035.7692332
ATCTCGT1950.035.7692337
GCGATCT1950.035.7692334
TATGCCG1950.035.7692343
ATAGCGA1950.035.7692331
ACGAGAC1950.035.7692322
AGCGATC1950.035.7692333
CTCGTAT1950.035.7692339
CACATAG1900.035.52631428
ACACATA1900.035.52631427
GACACAT1900.035.52631426
CATAGCG1900.035.52631430
AGACACA1900.035.52631425
GAGACAC1900.035.52631424
GTATGCC2000.034.87542
CCACGAG2000.034.87520
CACGAGA2000.034.87521
CCCACGA2000.034.87519
TCTCGTA2000.034.87538
Produced by FastQC (version 0.11.2)