FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_69.35100000046fed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_69.35100000046fed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32934
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC1079332.77160381368798No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT15854.812655614258821No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT2690.8167850853221594RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG1680.5101111313536163No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT1330.4038379789882796No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA880.26720106880427524No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT870.26416469302240847No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGGC820.24898281411307463No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA760.23076455942187407No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT750.22772818364000727No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA660.2004008016032064No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCCCGTATGCCGTC600.18218254691200583No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT530.16092791643893847No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA520.15789154065707173No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC520.15789154065707173No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT510.15485516487520495No Hit
CTTATACGCATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC480.14574603752960466No Hit
CTTATACACATCTCCGAGCCCATGAGACACATAGCGATCTCGTATGCCGTC470.1427096617477379No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATTTCGTATGCCGTC470.1427096617477379No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA460.13967328596587114No Hit
ATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCTTC460.13967328596587114Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTGTACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC440.13360053440213762No Hit
CTTAAACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC430.13056415862027085No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA410.12449140705653731No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT400.12145503127467056No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC390.11841865549280378No Hit
CTTATACACATCTCCGCGCCCACGAGACACATAGCGATCTCGTATGCCGTC380.11538227971093704No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA380.11538227971093704No Hit
CTTATACACTCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT380.11538227971093704No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC360.1093095281472035No Hit
CTTATACACATCTCCGAGCCCACGAGACACATAGCGAACTCGTATGCCGTC360.1093095281472035No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGG340.10323677658346997No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT340.10323677658346997No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG330.1002004008016032No Hit
CTTATATACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC330.1002004008016032No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC330.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13000.042.92307345
ATGCCGT14950.037.17391644
GTATGCC15500.036.2903242
CGTATGC15500.036.2903241
GAGACAC15200.036.2664524
TCGTATG15450.036.2621440
CGAGACA15100.036.2086123
ATCTCGT15550.036.17363437
TATGCCG15550.036.17363443
GATCTCG15550.036.17363436
AGACACA15400.036.0876625
CCACGAG15300.036.0294120
CACGAGA15300.036.0294121
ACGAGAC15300.036.0294122
GACACAT15550.036.0289426
GCCCACG15500.036.018
AGCGATC15500.036.033
CGATCTC15500.036.035
CTCGTAT15650.035.9424939
GCGATCT15400.035.94155534
Produced by FastQC (version 0.11.2)