H23LNBCXX_l01n01_bir_0120_pe_68.35100000046fc3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_68.35100000046fc3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285443
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC	24159	8.463686270113472	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT	4892	1.713827279001412	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	957	0.33526833728625327	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	825	0.28902442869504597	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	822	0.28797343077251847	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG	787	0.2757117883430317	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA	672	0.23542353464614652	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	576	0.20179160112526845	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	567	0.19863860735768613	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	547	0.19163195454083654	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	508	0.17796898154797983	No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC	506	0.17726831626629483	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	469	0.1643060085551231	No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC	440	0.15414636197069118	No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT	414	0.1450377133087867	RNA PCR Primer, Index 17 (95% over 21bp)
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG	388	0.13592906464688223	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	381	0.13347673616098485	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	375	0.13137474031592997	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG	332	0.11631043675970335	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT	300	0.10509979225274398	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC	295	0.10334812904853159	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	2695	43.580364	45
GCCGTCT	85	39.70557	45
ATGCCGT	3215	36.461613	44
TATGCCG	3325	35.255363	43
TAGCGAT	3295	35.23493	32
AGCGATC	3290	35.220093	33
GCGATCT	3290	35.220093	34
ATCTCGT	3320	35.17292	37
TCTCGTA	3320	35.105152	38
CGTATGC	3335	35.082188	41
GATCTCG	3310	35.075256	36
CTCGTAT	3330	35.067295	39
ATAGCGA	3325	35.052357	31
TCGTATG	3335	35.01472	40
CATAGCG	3330	34.999725	30
CGATCTC	3310	34.939304	35
CGAGACA	3365	34.903145	23
GTATGCC	3360	34.888123	42
CACGAGA	3380	34.881382	21
ACGAGAC	3380	34.814816	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content