FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_66.35100000046f80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_66.35100000046f80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144511
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC5035034.84163835278976No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT1885613.048141663956377No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG37522.596342146964591No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT5600.3875137532782972RNA PCR Primer, Index 17 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA2910.20136875393568657No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2480.17161323359467445No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA2330.1612334009175772No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT1990.13770578018282345No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT1820.12594196981544659No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1750.12109804789946785No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1710.1183300925189086No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA1650.1141781594480697No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT1530.10587429330639189No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC1470.101722360235553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCC207.01387E-444.9986159
ACTCCGA207.01387E-444.99861511
TGCCGTC53250.043.68879745
TAGCGAC301.1351953E-437.49884432
CGTCTTC301.1351953E-437.49884445
TAACTGA250.002101348535.998898
TTGTACT401.5478317E-533.7606434
ATGCCGT71550.032.51472544
CGGGACG352.8087763E-432.1418718
GGGACGC352.8087763E-432.1418719
GCCGTCT1500.031.49903345
CGATCTC74900.030.73002835
TATGCCG75650.030.72278243
TCTCGTA75000.030.68905638
GATCTCG74900.030.6699536
GCGATCT75050.030.6686134
CTCGTAT75300.030.65642739
ATCTCGT74950.030.64948837
TCGTATG75400.030.64560940
CATAGCG75850.030.64177530
Produced by FastQC (version 0.11.2)