FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_65.35100000046f66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_65.35100000046f66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122650
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTC1325110.803913575214024No Hit
TCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGT17031.3885038728088055No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCG3720.30330207908683243No Hit
TTATACACATCTCCGAGCCCACGAGACACATAGCGATCTCGTATGCCGTCT2580.2103546677537709RNA PCR Primer, Index 17 (95% over 21bp)
GTCTAATATCCATAATCTACAAGTAACTCAAAGAAATCCACAACCAAAACA1880.15328169588259274No Hit
ATGCTATAGATCATCTAGAGGCTGATTATGAAAGGAATGCACTTGAGAAAG1810.14757439869547492No Hit
ATGTATGGTATGACACTAATAAAGATGGTATTCAAGGTGATGATGAAAAAG1800.14675907052588666No Hit
AACTAACATACACAAAGTCTGTGATGAAAATAAAACGGTTGTTATTCCTTT1710.13942111699959234No Hit
ATACAGACATACACACAAATACATACACACACACACATGCACACATACACT1630.13289849164288625No Hit
AACAACAACAAAGGTTTTGTTTTTTAGGCAACTGAAACTGCTGTAAAACAT1530.12474520994700367No Hit
AATACAAAGAGCTAAACAAACAACAAAATGCTTAAATCACCACTTTTGCTT1440.11740725642070933No Hit
GATGATATCTCTTGTAGCATACAAATCCATGGATTGTACATTCTTATTATC1420.11577660008153283No Hit
TACATATGGAGAGAGAGATAAATGGATGGATGAACAAATCAGCCAAACAAT1390.11333061557276805No Hit
AGTTTACTGAGAATGATGATTTCCAATTTCATCCATGTCCCTACAAAGGAC1340.10925397472482674No Hit
CGTTTACTCTATGTTTTCTAGTTTGTGCTCATAGAGATGTTCATAGTAGTC1270.10354667753770892No Hit
AGAGTAAACAATCCCAAAATAAAGAAGAGATGGTGACAGAACAATGTAGTT1240.10110069302894414No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA207.008952E-444.9995939
AAGAGCC207.008952E-444.9995926
CCTAGAT207.008952E-444.9995913
CGCATTT207.008952E-444.999598
CCTAAGG207.008952E-444.9995924
GGCTTTC253.870597E-544.999597
ACAGGTT207.008952E-444.999595
ACAGGAC253.870597E-544.9995912
TCGCTTA207.008952E-444.999598
TACTGGG207.008952E-444.999592
GATGAGC207.008952E-444.9995931
CAGTCAG253.870597E-544.9995934
TAGGAGG207.008952E-444.999595
ATCGCTT207.008952E-444.999597
CGCTTAA207.008952E-444.999599
TGCCGTC13100.043.2820545
GGTATGA501.0641088E-940.4996347
CTCTGAG403.4249024E-739.3746434
TGTTAAC356.203156E-638.5710754
ATGCCGT14900.037.9023444
Produced by FastQC (version 0.11.2)