FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_64.35100000046f4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_64.35100000046f4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205773
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC180618.777147633557366No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT16070.7809576572242228No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT10020.48694435129973324No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5340.2595092650639297No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCTTC4810.23375272752013143RNA PCR Primer, Index 20 (95% over 23bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4670.22694911382931676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.21625772088660805No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4130.20070660387903175No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4120.20022063147254499No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3520.1710622870833394No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3270.15891297692117043No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2980.14481977713305438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.1351003290033192No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.13169852215791186No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCTT2500.12149310162168991RNA PCR Primer, Index 20 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.11420351552438852No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG2270.11031573627249446No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2150.10448406739465334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.10059628814275925No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2060.10011031573627249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21550.038.31496445
ATGCCGT22700.036.37390544
CTCGTAT22600.036.3357539
TATGCCG22600.036.3357543
TGGTCAT22800.036.32187332
GTCATCT22700.036.2836134
ATCTCGT22700.036.2747937
GTATGCC22650.036.2555442
CTGGTCA22850.036.2423931
CGAGACT22850.036.23358523
CGTATGC22750.036.19506541
TCTCGTA22750.036.19506538
AGACTCC22800.036.1244725
CCACGAG23050.036.11440720
GCCAGCC250.002101370436.00602729
TCGTTAC250.002103879635.99727620
TATATAT250.002103879635.9972762
CGGGATG250.002103879635.9972764
GGTCATC22900.035.9667233
CCTGGTC23050.035.92792530
Produced by FastQC (version 0.11.2)