FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_62.35100000046f09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_62.35100000046f09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279349
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC247778.869550275819853No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT33601.2027965018668403No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7890.2824423928490884No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG7450.26669148627702266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.22516636895066744No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5050.1807774504293912No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.16860629535097674No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4690.16789034505224645No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.1646685687079603No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.14462196034351293No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3780.13531460646001953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.1231434513816051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.117415848991763No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3250.1163419235436676No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.11491002294620707No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3100.11097229630319062No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT3000.10739254480953932No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2940.10524469391334855No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2920.10452874361461828No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2910.10417076846525315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCAA207.015984E-445.01056729
CGTAGAT207.022178E-445.0025062
TGCCGTC27250.044.0037545
ATGCCGT30450.039.3793844
GTATGCC31400.038.11631842
CATCTCG31500.038.06673436
TATGCCG31500.038.06673443
CGTATGC31450.038.0557241
CTCGTAT31550.038.00640539
AGACTCC31350.037.9686225
CACGAGA31650.037.89310521
ACGAGAC31650.037.89310522
ATCTCGT31650.037.88632237
CCACGAG31800.037.84910620
CGAGACT31600.037.8106523
TCGTATG31700.037.75559640
GACTCCT31300.037.74172226
CTGGTCA31550.037.7346931
CTCCTGG31350.037.6882728
TCTCGTA31900.037.5894138
Produced by FastQC (version 0.11.2)