FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_60.35100000046ec4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_60.35100000046ec4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388949
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTC235726.0604346585284965No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGT33150.8522968306898847No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA8150.2095390398227017No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC8010.20593959619384544No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC7070.18177190325723938No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6560.16865964432354885No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCG6500.16711702562546762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA5970.15349056045908333No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA5600.14397774515424902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.13497913608210846No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCT5210.13395072361672097No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5020.12906576440613035No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4640.11929584598494919No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4520.1162106085887867No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.11466798989070548No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4330.1113256493781961No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4280.11004013379646174No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA4090.10515517458587116No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.10129862784066805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29050.042.7504545
ATGCCGT32550.038.1536344
TATGCCG34050.036.40677643
GTATGCC34050.036.40677642
CCTGGTC33850.036.36070330
CTCCTGG33800.036.3479228
CACGAGA34150.036.3001721
TCCTGGT33850.036.2942329
CGTATGC34050.036.2746341
ACGAGAC34250.036.25987222
TCTCGTA33850.036.223138
CGAGACT34200.036.18131623
CATCTCG34000.036.12946336
CCACGAG34250.036.12849820
ATCTCGT33950.036.11640537
TCGTATG34150.036.10252840
CTGGTCA34200.036.05438231
AGACTCC34150.036.04128325
GGTCATC34100.036.0281433
GACTCCT34250.036.00174726
Produced by FastQC (version 0.11.2)