FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_56.35100000046e31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_56.35100000046e31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87135
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC52666.043495725024387RNA PCR Primer, Index 33 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT10021.1499397486658633RNA PCR Primer, Index 33 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTTC4170.47856773971423655RNA PCR Primer, Index 33 (95% over 24bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.4716818729557583No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.4636483617375337No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3910.4487289837608309No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3840.4406954725426063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.3454409823836575No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2930.3362598267056866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.3201928042692374No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.31330693751075916No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.28920640385608537No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2310.26510587020141163No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT2030.2329718253285132No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2010.23067653640902047No Hit
TATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTT1990.22838124748952773RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.22723360302978135No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1830.2100189361335858No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.18018018018018017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.18018018018018017No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.1744419578814483No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.16067022436449188No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1220.1400126240890572No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1080.12394560165260803No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA960.11017386813565158No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA960.11017386813565158No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGCC206.997397E-445.041
CGTCTTC601.5097612E-1037.50000445
ACTGTCC250.002096457935.9999968
TGCCGTC8650.033.55491345
CACGAGA8750.033.1714321
CGAGACG8800.032.98295623
ACGAGAC8850.032.79660822
CCACGAG8950.032.6815720
GGAAGGC9050.032.5690629
GAGACGG9000.032.524
CCCACGA9100.032.3901119
ATGCCGT8900.032.3595544
AGGCGAT9050.032.32044232
AGACGGA9050.032.32044225
GCCCACG9200.032.2826118
GCGATCT9000.032.2534
CTCGTAT8800.032.2159139
TCGTATG8800.032.2159140
GTATGCC8950.032.17877242
TTCAAAG352.7991677E-432.14285717
Produced by FastQC (version 0.11.2)