FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_54.35100000046e0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_54.35100000046e0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202520
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC174198.601125814734347RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT17910.8843571005332806No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11150.5505629073671736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.4182302982421489No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8090.3994667193363618No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7460.3683586806241359No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7400.3653960102705906No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6880.3397195338731977No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.3372506419119099No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.27404700770294294No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5020.24787675291329253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.24343274738297455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.23306340114556587No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG4540.22417539008492987No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4440.21923760616235433No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.21825004937783923No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4020.19849891368753703No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.1585028639146751No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3040.15010863124629664No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.14813351767726646No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2900.1431957337546909No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.13578905787082757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.11307525182698006No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2210.10912502468891962No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2180.10764368951214694No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT2160.10665613272763184RNA PCR Primer, Index 33 (100% over 21bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2030.10023701362828362No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA207.023688E-444.99456840
CACTTCG207.023688E-444.9945685
TGCCGTC19500.043.37937545
ATGCCGT21700.038.9814744
GTATGCC22100.038.2759242
CGTATGC22150.038.1895241
TATGCCG22150.038.1895243
CTCGTAT22000.038.14312439
GGAAGGC21850.038.10549529
CGATCTC21800.038.0896735
AGGCGAT21750.038.07377232
ATCTCGT22000.038.04086337
CACGAGA22250.038.0272721
GATCTCG21900.038.00911336
GACGGAA21850.038.00250626
GAAGGCG21850.038.00250630
GAGACGG22150.037.99576624
AGACGGA22050.037.96397425
TCTCGTA22050.037.954638
CGGAAGG21900.037.91574528
Produced by FastQC (version 0.11.2)