FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_53.35100000046def.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_53.35100000046def.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254250
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC202937.981514257620452RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT62852.471976401179941No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG8920.35083579154375616No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4930.19390363815142575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA4920.1935103244837758No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4790.18839724680432646No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA4050.1592920353982301No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4000.15732546705998035No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4000.15732546705998035No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3780.1486725663716814No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3770.14827925270403147No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3620.14237954768928218No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT3620.14237954768928218RNA PCR Primer, Index 33 (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC3560.1400196656833825No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3540.1392330383480826No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3540.1392330383480826No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3470.13647984267453295No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG207.018529E-445.00570329
CGTACGC207.0253346E-444.99685325
ACGCTAG207.0253346E-444.99685328
GTACGCT207.0253346E-444.99685326
TGCCGTC22100.043.8770245
GGCTAAT356.2321014E-638.56873311
TCGTACG250.002104752235.99748224
GTGAGGG250.002104752235.99748211
ATGCCGT28300.034.1848944
TATGCCG29350.032.88526543
GATCTCG29100.032.7812136
AGGCGAT29350.032.7383932
AAGGCGA29450.032.70363631
CGTATGC29450.032.69720541
TCGTATG29400.032.6762940
GGCGATC29300.032.6406633
GCGATCT29250.032.6195234
GGAAGGC29600.032.61393429
GTATGCC29550.032.58655542
GAAGGCG29650.032.55893330
Produced by FastQC (version 0.11.2)