H23LNBCXX_l01n01_bir_0120_pe_52.35100000046dc5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_52.35100000046dc5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	214917
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC	11897	5.535625380961022	RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT	1112	0.5174090462829837	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	760	0.35362488774736295	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.27731635933872145	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	594	0.27638576752886	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	576	0.26801044124010664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	564	0.26242689038093775	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	531	0.2470721255182233	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	465	0.21636259579279443	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	420	0.19542428007091112	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT	419	0.19495898416598034	RNA PCR Primer, Index 33 (100% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	397	0.18472247425750407	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	357	0.16611063806027443	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	341	0.15866590358138258	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	335	0.15587412815179813	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.14935998548276777	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	309	0.14377643462359888	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	250	0.11632397623268517	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	244	0.11353220080310072	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	237	0.11027512946858554	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG	235	0.10934453765872407	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	234	0.10887924175379332	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	223	0.10376098679955519	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	223	0.10376098679955519	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	219	0.10189980317983222	No Hit
ATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTTC	217	0.10096921136997074	RNA PCR Primer, Index 33 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGTCT	20	7.021728E-4	44.998375	11
TTGGGTC	20	7.021728E-4	44.998375	10
TGCCGTC	1335	0.0	42.807438	45
GCCGTCT	45	1.9164872E-8	39.998554	45
CTCGTAT	1455	0.0	38.658394	39
ATCTCGT	1465	0.0	38.54809	37
TCTCGTA	1460	0.0	38.526	38
CGATCTC	1470	0.0	38.416977	35
TATGCCG	1485	0.0	38.331947	43
GCGATCT	1485	0.0	38.331947	34
ATGCCGT	1485	0.0	38.331947	44
GATCTCG	1475	0.0	38.28675	36
CGTATGC	1470	0.0	38.26392	41
TCGTATG	1470	0.0	38.26392	40
GGCGATC	1495	0.0	38.075542	33
GTATGCC	1495	0.0	37.925045	42
GGAAGGC	1490	0.0	37.901314	29
GAGACGG	1485	0.0	37.87742	24
CGAGACG	1485	0.0	37.87742	23
AAGGCGA	1500	0.0	37.798634	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content