FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_51.35100000046dac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_51.35100000046dac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211883
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC134516.348314871886843RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT15900.7504141436547529No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8050.37992665763652583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.26760051537877033No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5520.2605211366650463No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5230.24683433781851305No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5160.24353062775210846No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4810.2270120774200856No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4660.21993269870636153No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.1746246749385274No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3650.1722648820339527No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3570.1684892133866332No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG3480.16424158615839873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.15905004176833443No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT3080.1453632429218012RNA PCR Primer, Index 33 (100% over 21bp)
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.14017169853173686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.14017169853173686No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2550.12034943813330942No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2340.1104383079340957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.10666263928677619No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2240.10571872212494632No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2170.10241501205854174No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2160.10194305347762679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15300.042.79097445
ACACTAC301.13411494E-437.5149546
ATGCCGT17800.036.78100644
CGGAAGG18100.036.04707728
GTATAGT250.002094262536.0313721
GCGATCT18050.036.02228534
CGATCTC18050.036.02228535
CACTGGC250.002103997435.9973567
CGTTCTC250.002103997435.99735643
GATAGGT250.002103997435.9973567
CGTATGC18150.035.94777341
GAGACGG18300.035.89900624
GATCTCG18050.035.89764436
TATGCCG18250.035.87407743
GTATGCC18200.035.84901442
ATCTCGT18150.035.8238237
CGAGACG18350.035.80118623
GGCGATC18200.035.72539533
AGGCGAT18150.035.6998632
GGAAGGC18300.035.65312229
Produced by FastQC (version 0.11.2)