H23LNBCXX_l01n01_bir_0120_pe_49.35100000046d68.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_49.35100000046d68.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132772
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTC	10036	7.558822643328413	RNA PCR Primer, Index 33 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGT	1554	1.170427499774049	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCG	313	0.23574247582321575	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCT	282	0.2123941794956768	RNA PCR Primer, Index 33 (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGAAGGCGATCTCGTATGCCGTCTTC	143	0.1077034314463893	RNA PCR Primer, Index 33 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGT	20	6.9994613E-4	45.01469	6
TGCCGTC	1120	0.0	41.984497	45
CCTTAGA	35	6.192722E-6	38.584023	2
ATGCCGT	1300	0.0	36.17126	44
CCGTCTG	25	0.0021009338	35.99819	22
GCGATCT	1290	0.0	35.754017	34
CTCGTAT	1325	0.0	35.658585	39
GACGGAA	1320	0.0	35.62321	26
AGACGGA	1315	0.0	35.587566	25
AGGCGAT	1290	0.0	35.579605	32
GGCGATC	1290	0.0	35.579605	33
CGGAAGG	1310	0.0	35.55165	28
ACGGAAG	1310	0.0	35.55165	27
CGTATGC	1330	0.0	35.524532	41
TATGCCG	1330	0.0	35.524532	43
GAGACGG	1330	0.0	35.524532	24
TCGTATG	1330	0.0	35.524532	40
CCCACGA	1325	0.0	35.48878	19
GATCTCG	1300	0.0	35.47899	36
CGATCTC	1300	0.0	35.47899	35