FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_48.35100000046d4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_48.35100000046d4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151807
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC118227.787519679593167No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT12230.8056281989631572No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT7200.47428642947953653Illumina PCR Primer Index 7 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3270.21540508672195616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.19103203409592442No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.18773837833564985No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.1778574110548262No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG2480.16336532570961812No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.14755577806030026No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2130.1403097353876962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA2080.13701607962742166No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA2070.13635734847536674No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2050.13503988617125692No Hit
ATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCTTC2010.13240496156303727Illumina PCR Primer Index 7 (95% over 23bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1950.1284525746507078No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1730.11396048930549973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.10671444663289571No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.10342079087262115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATAT207.0139323E-445.01
TGCCGTC13500.040.3333345
GCCGTCT1350.040.00000445
ATGCCGT15000.036.1544
GAGACGT15500.034.98386824
ATCTCGT15650.034.936137
GTTAGGT15400.034.9188329
CGTTAGG15400.034.9188328
GGTCATC15600.034.90384733
TATGCCG15550.034.8713843
AGACGTT15550.034.8713825
CGAGACG15550.034.8713823
ACGAGAC15650.034.7923322
CTCGTAT15600.034.75961739
GTATGCC15600.034.75961742
CGTATGC15550.034.7266941
TTAGGTC15500.034.69354630
GACGTTA15500.034.69354626
CATCTCG15700.034.68152636
CACGAGA15700.034.68152621
Produced by FastQC (version 0.11.2)