FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_47.35100000046d25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_47.35100000046d25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307779
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC287869.352814844417585No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT40831.3266012301034185No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9820.31906010481546826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.2937172451661745No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG8790.28559453374011873No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6060.19689452496759038No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5930.1926707150260414No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.1913710811978725No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.18649745434223908No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5690.18487291205702794No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT5380.17480074988871885Illumina PCR Primer Index 7 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.16700294691970538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.1523820663528051No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4680.15205715789576288No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4420.14360953801266493No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4080.13256265047322915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.12866374898872243No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3930.12768902361759574No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3700.12021612910562449No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3400.11046887539435764No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3360.10916924156618873No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.10624506545280867No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3210.1042956147105553No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3200.10397070625351307No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3130.10169634705421747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC207.028353E-444.9959377
TGCCGTC31350.042.91478745
ATGCCGT36100.037.26810544
CACGAGA37150.036.33588421
CGTTAGG36800.036.2535228
TAGGTCA36700.036.229731
CGAGACG37200.036.22656623
CGTATGC37200.036.1660941
AGACGTT37150.036.15420525
GTTAGGT36850.036.1432829
GAGACGT37100.036.1422924
TCTCGTA37100.036.1422938
CCACGAG37350.036.1413220
AGGTCAT36800.036.1312532
CTCGTAT37050.036.1303439
GACGTTA37000.036.11836226
TATGCCG37250.036.11754243
CATCTCG36950.036.1063536
ACGAGAC37450.036.10488522
ATCTCGT37150.036.03308537
Produced by FastQC (version 0.11.2)