H23LNBCXX_l01n01_bir_0120_pe_46.35100000046d0b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_46.35100000046d0b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84983
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC	8920	10.496216890436912	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT	1391	1.636797947824859	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG	296	0.34830495510866877	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	244	0.2871162467787675	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206	0.24240142146076277	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	151	0.17768259534259792	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	141	0.16591553604838616	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	136	0.16003200640128026	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	135	0.15885530047185906	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.1529717708247532	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.1529717708247532	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	127	0.14944165303648965	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	119	0.14002800560112022	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.12355412258922374	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	104	0.12237741665980255	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	101	0.118847298871539	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.118847298871539	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100	0.11767059294211783	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	92	0.10825694550674841	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	90	0.10590353364790606	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT	88	0.1035501217890637	Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	86	0.10119670993022135	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	945	44.044167	45
ATGCCGT	1055	39.23863	44
GACGTTA	1085	38.361046	26
GAGACGT	1085	38.361046	24
CGTTAGG	1080	38.330322	28
CGAGACG	1080	38.330322	23
ACGTTAG	1090	38.185074	27
ATCTCGT	1085	38.15369	37
CGTATGC	1085	38.15369	41
ACGAGAC	1085	38.15369	22
CTCGTAT	1080	38.122005	39
CATCTCG	1080	38.122005	36
GGTCATC	1080	38.122005	33
TCTCGTA	1080	38.122005	38
TCGTATG	1080	38.122005	40
AGACGTT	1095	38.010715	25
TTAGGTC	1090	37.97867	30
GTTAGGT	1090	37.97867	29
CACGAGA	1090	37.97867	21
TATGCCG	1090	37.97867	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content