FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_45.35100000046cd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_45.35100000046cd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293041
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC5648219.274436000423147No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT93313.1841960681269854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG42671.4561102371340529No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT11910.40642776949300613No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT10650.36343037322422456No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT9970.340225429206152No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG9840.33578918990857937No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG9320.3180442327182886No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT9220.31463174095092494No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA9080.3098542524766159No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA8250.28153057080749794No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC7410.25286563996164363No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC7400.25252439078490724No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA7360.2511593940779618No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6920.2361444303015619No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA6410.21874072228800748No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA6250.2132807354602257No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT6240.21293948628348935No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5320.1815445620237441No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5300.18086206367027138No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA5210.17779082107964414No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5040.17198958507512602No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5030.17164833589838965No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA4760.1624346081265079No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4710.1607283622428261No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT4620.15765711965219886No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG4600.1569746212987261No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC4560.15560962459178068No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATC4550.15526837541504432No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA4480.15287963117788977No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4380.1494671394105262No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT4360.14878464105705344No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC4280.14605464764316256No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT4220.14400715258274438No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4100.13991216246190805No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTCTGTCTCTT4070.13888841493169898No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC4060.13854716575496262No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4040.1378646674014899No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG4030.13752341822475353No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3990.1361584215178081No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3980.1358171723410717No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTA3870.13206343139697177No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA3790.12933343798308086No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATC3680.1255796970389809No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3650.1245559495087718No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC3620.12353220197856273No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA3560.12148470691814456No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3550.1211434577414082No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC3460.11807221515078095No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3450.11773096597404459No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTC3420.11670721844383551No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3410.11636596926709913No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG3370.1150009725601537No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT3350.11431847420668097No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG3340.11397722502994462No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAT3260.11124723161605372No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG3240.11056473326258101No Hit
GCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG3230.11022348408584463No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA3220.10988223490910828No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCCTGTCTCTTATACACATCT3180.10851723820216284No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3170.10817598902542647No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC3160.10783473984869012No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA3140.10715224149521739No Hit
GCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC3130.10681099231848103No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3070.10476349725806286No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTA3060.10442224808132651No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA3040.10373974972785378No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC2960.10100975631396289No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA2960.10100975631396289No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCT207.0259866E-444.99829518
GTAATCC207.0259866E-444.99829529
TTGCCCG253.8845395E-544.9982916
TGCCGTC60950.044.70298445
ATGCCGT70400.038.6704144
GAAAGTA356.226688E-638.576552
GCCGTCT356.2339896E-638.56996545
CGTTGCC301.1381011E-437.49857714
GTTGCCC301.1381011E-437.49857715
TATGCCG74300.036.64060243
GTATGCC74450.036.56678442
CGTATGC74500.036.5120441
TCGTATG74350.036.49491540
CTCGTAT74500.036.4214439
CATCTCG74400.036.3796736
ATCTCGT74550.036.3668337
TCTCGTA74550.036.3668338
GGTCATC74150.036.32026733
AGGTCAT74150.036.28992532
GTCATCT74150.036.28992534
Produced by FastQC (version 0.11.2)