FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_43.35100000046c90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_43.35100000046c90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157340
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC2347914.922460912673191No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT66614.2335070547858145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG9090.5777297572136774No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT4560.2898182280411847Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC2600.1652472352866404No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2030.1290199567814923No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2030.1290199567814923No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA1920.1220287275962883No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1710.10868183551544426No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1600.10169060633024025No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC1580.10041947375111224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC27200.043.5110345
ACGGGAT301.1354481E-437.5000045
GTGATGG250.002101596436.012
GAGCGTG250.002101596436.011
GTGACTG250.002101596436.033
ATGCCGT33450.035.44843344
GTATGCC33950.034.9263642
TATGCCG34050.034.8898743
ATCTCGT33600.034.88839337
TCTCGTA33800.034.88165738
CGTATGC33950.034.8600941
CTCGTAT33950.034.79381639
CATCTCG33600.034.75446736
CACGAGA34050.034.6916321
GAGACGT34050.034.6916324
TCGTATG34050.034.6916340
CGTTAGG33800.034.68195328
CGAGACG34100.034.64076223
GTTAGGT33800.034.61538329
ACGTTAG33950.034.59499427
Produced by FastQC (version 0.11.2)