FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_41.35100000046c5d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_41.35100000046c5d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143802
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTC2373816.50741992461857No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGT70394.89492496627307No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCG8720.6063893408992921No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCT7710.5361538782492594Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCTTC2000.13908012405947065Illumina PCR Primer Index 7 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1580.10987329800698183No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1560.10848249676638712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC28400.040.95950745
CGTCTTC301.1349622E-437.50000445
GACTCAT301.1349622E-437.5000049
GCCGTCT2350.035.42553345
ATGCCGT36600.031.78278744
ATCTCGT37050.031.09311737
ACGAGAC37500.031.08000222
CATCTCG36850.031.078736
CGTATGC37450.031.06141341
CACGAGA37550.031.03861621
CTCGTAT37200.031.02822739
TCTCGTA37150.031.00942238
TCGTATG37350.030.96385440
GAGACGT37600.030.937524
CGAGACG37600.030.937523
CCACGAG37600.030.937520
AGACGTT37650.030.89641425
GTATGCC37650.030.89641442
GACGTTA37600.030.87765926
ACGTTAG37550.030.85885627
Produced by FastQC (version 0.11.2)