FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_39.35100000046c19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_39.35100000046c19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240593
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC2480410.30952687734057No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT29041.2070176605304395No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10690.4443188288936087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.4056643376989355No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8410.3495529795131197No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7620.31671744398216073No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7520.3125610470795077No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7280.30258569451314044No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6820.2834662687609365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.2414866600441409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.23483642499989607No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5470.22735491057512067No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT5320.22112031522114112No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5090.21156060234503912No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4750.19742885287601883No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG4500.18703786061938626No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.1778937874335496No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4220.1753999492919578No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.1367454580972846No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3270.135914178716754No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.1338359802654275No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.12635446584065207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.12510754676985614No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2570.10681940039818283No Hit
ATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTTC2530.1051568416371216RNA PCR Primer, Index 24 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAC207.0178375E-445.0056155
TGCCGTC29200.042.99345
ATGCCGT31800.039.54859544
CGAGACG32400.039.02453623
GGCATAG32200.038.9955529
ACGAGAC32400.038.95509722
CGGCATA32300.038.94448528
ACGGCAT32300.038.94448527
TAGGATC32050.038.8972133
GAGACGG32400.038.8856624
TATGCCG32350.038.87621343
GCATAGG32300.038.87481730
CATAGGA32250.038.8653131
CGTATGC32250.038.8572341
CTCGTAT32150.038.8381439
AGACGGC32450.038.82573725
CCACGAG32450.038.82573720
GACGGCA32400.038.82428726
GTATGCC32400.038.8162242
ATCTCGT31950.038.79958737
Produced by FastQC (version 0.11.2)