FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_35.35100000046b91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_35.35100000046b91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157245
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC2276714.478679767242204No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT69984.4503799802855415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG6650.42290692867817736No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT5720.36376355369010144No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2260.1437247607237114No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1990.12655410346910873No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1980.1259181532004197No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC1690.10747559540843907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23700.043.1943745
GGGAGCA301.13572816E-437.4984117
GGAGTTG250.002098747836.009933
CCCTAGA250.00210202935.99847432
GCCGTCT1050.034.2842645
ATGCCGT30800.033.31027644
GATCTCG31250.032.61461636
TCTCGTA31300.032.5625238
CTCGTAT31400.032.53046839
CGGCATA31500.032.4986228
CGTATGC31650.032.48677441
ATAGGAT31450.032.47875232
AGACGGC31600.032.4669825
GTATGCC31600.032.4669842
GACGGCA31600.032.4669826
GAGACGG31550.032.4471224
ACGGCAT31550.032.4471227
CGAGACG31550.032.4471223
GCATAGG31550.032.4471230
ACGAGAC31700.032.43553522