FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_34.35100000046b77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_34.35100000046b77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73980
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC1498320.252771019194377No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT57457.7656123276561235No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT4930.6663963233306299No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG4680.6326034063260341No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1140.15409570154095703No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1060.14328196809948635No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG1010.1365233846985672No Hit
TATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTT920.12435793457691267RNA PCR Primer, Index 24 (95% over 21bp)
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC890.12030278453636117No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA890.12030278453636117No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC860.11624763449580967No Hit
ATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTTC770.10408218437415519RNA PCR Primer, Index 24 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC740.10002703433360369No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA740.10002703433360369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15850.042.7287145
GCCGTCT700.038.57142645
ATGCCGT21800.031.06651544
ATCTCGT22300.030.36995537
AGGATCT22350.030.30201334
TCTCGTA22350.030.30201338
GATCTCG22300.030.26905836
TATGCCG22400.030.23437343
CTCGTAT22400.030.23437339
GGATCTC22350.030.20134235
GTATGCC22450.030.16703842
TCGTATG22450.030.16703840
CGTATGC22500.030.141
TAGGATC22450.030.06681633
CATAGGA22550.030.03325831
ATAAAAC300.005116534430.0000023
ATAGGAT22550.029.93348132
ACGAGAC22550.029.93348122
CCACGAG22650.029.90066320
GGCATAG22600.029.86725829
Produced by FastQC (version 0.11.2)