FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_33.35100000046b6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_33.35100000046b6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115479
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTC3951434.217476770668256No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGT1499612.985910858251284No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG13631.1803011803011803No Hit
TTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT7460.6460049013240502No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCG1660.1437490799192927No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA1650.14288312160652586No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA1630.14115120498099223No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1550.13422353847885762No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT1380.11950224716182163No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1330.11517245559798753No Hit
ATACACATCTCCGAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTTC1160.10045116428095151RNA PCR Primer, Index 24 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC41650.043.2172945
GCCGTCT1600.037.96875445
ATGCCGT57550.031.23805444
TATGCCG59250.030.30379743
CCACGAG59600.030.27684820
CACGAGA59550.030.26448421
ACGAGAC59550.030.26448422
GTATGCC59200.030.25337842
AGACGGC59200.030.25337825
CCCACGA59650.030.25146919
CGAGACG59400.030.2272723
GAGACGG59400.030.2272724
ATCTCGT58900.030.21646937
CGTATGC59200.030.21537241
TCGTATG59100.030.19035540
GCATAGG59100.030.19035530
GACGGCA59200.030.17736426
CGGCATA59200.030.17736428
TCTCGTA59000.030.16525338
GATCTCG59000.030.16525336
Produced by FastQC (version 0.11.2)