FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_31.35100000046b27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_31.35100000046b27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325850
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC231797.113395734233544No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT32661.0023016725487186No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA7360.22587079944759858No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG6760.2074574190578487No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6060.18597514193647385No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5630.1727788859904864No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5320.163265306122449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.15712751265919903No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4830.1482277121374866No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4530.13902102194261165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA4500.13810035292312414No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4400.13503145619149914No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4370.13411078717201166No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA3890.11938008286021176No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT3870.11876630351388676No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3660.1123216203774743No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT3630.1114009513579868No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.10741138560687433No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG253.886043E-544.9972345
TGCCGTC23500.043.56115745
ATGCCGT26250.038.82618744
AGACTCG27150.037.46201325
GTATGCC27150.037.46201342
GCATCTC26850.037.45580335
CGTATGC27100.037.44809741
CTCGTAT27050.037.4341339
GACTCGT27200.037.39314726
TATGCCG27300.037.338643
GTGCATC26950.037.32254433
ACTCGTG27100.037.28203227
CGAGACT27400.037.27873623
TCGTGTG26950.037.2390529
CACGAGA27500.037.2249921
ACGAGAC27500.037.2249922
CTCGTGT27150.037.2133728
CATCTCG27150.037.2076636
ATCTCGT27300.037.1737537
TCTCGTA27200.037.14496238