FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_30.35100000046b0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_30.35100000046b0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217604
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC93304.287605007260896No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT10820.4972335067370085No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4290.19714711126633702No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG3730.1714122902152534No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3130.14383926766052096No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.1410819654050477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.13832466314957448No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.12315950074447161No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.10707523758754435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9600.043.3593745
ATGCCGT10900.038.18807244
CGTGTGC11150.036.72645630
CTCGTGT11300.036.4380528
TCGTGTG11300.036.4380529
TATGCCG11450.036.3537143
GACTCGT11450.036.3537126
ACTCGTG11350.036.2775327
GTGTGCA11300.036.23893731
ATCTCGT11450.036.15720437
CGTTCTG250.002103364836.09
CCCGTTA250.002103364836.029
CACGAGA11450.035.96069721
CCACGAG11450.035.96069720
AGACTCG11600.035.8836225
CGTATGC11600.035.8836241
ACGAGAC11550.035.84415422
CATCTCG11500.035.80434836
GCATCTC11450.035.7641935
CTCGTAT11600.035.68965539