H23LNBCXX_l01n01_bir_0120_pe_29.35100000046ae2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_29.35100000046ae2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	97279
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC	18912	19.44098931938034	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT	3994	4.105716547250691	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG	741	0.7617265802485634	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	577	0.5931393209222957	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	491	0.5047338068853504	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	476	0.4893142404835576	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	473	0.4862303272031991	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT	319	0.3279227788114598	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA	314	0.32278292334419556	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC	312	0.32072698115728987	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC	309	0.3176430678769313	No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT	281	0.2888598772602515	No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT	266	0.27344031085845866	No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT	266	0.27344031085845866	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA	227	0.23334943821379742	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT	211	0.2169019007185518	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	208	0.21381798743819325	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	202	0.20765016087747612	No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC	195	0.20045436322330615	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC	193	0.19839842103640046	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT	176	0.18092291244770198	No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT	162	0.16653131713936206	No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA	162	0.16653131713936206	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	151	0.15522363511138065	No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT	150	0.15419566401792784	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	148	0.15213972183102212	No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC	142	0.145971895270305	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	140	0.1439159530833993	No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT	132	0.13569218433577648	No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA	124	0.12746841558815364	No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC	101	0.10382508043873806	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG	101	0.10382508043873806	No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA	100	0.10279710934528521	No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTT	99	0.10176913825183234	No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG	99	0.10176913825183234	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCT	98	0.1007411671583795	No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTA	98	0.1007411671583795	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	2235	0.0	43.489933	45
ATGCCGT	2690	0.0	36.050186	44
TTTACCA	25	0.0020976579	36.000004	4
ACGAGAC	2770	0.0	35.577618	22
CCACGAG	2765	0.0	35.560577	20
CGAGACT	2765	0.0	35.560577	23
GCATCTC	2690	0.0	35.464684	35
CTCGTGT	2735	0.0	35.45704	28
CACGAGA	2780	0.0	35.449642	21
GAGACTC	2780	0.0	35.449642	24
ATCTCGT	2700	0.0	35.416668	37
ACTCGTG	2745	0.0	35.409836	27
GTGCATC	2715	0.0	35.38674	33
CGTGTGC	2735	0.0	35.37477	30
CATCTCG	2710	0.0	35.369003	36
TCGTGTG	2730	0.0	35.357143	29
TCTCGTA	2705	0.0	35.3512	38
GACTCGT	2790	0.0	35.322582	26
CCCACGA	2785	0.0	35.305206	19
GTGTGCA	2735	0.0	35.292503	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content