FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_25.35100000046a5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_25.35100000046a5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128912
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC1302810.106118902817427No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGT18441.4304331637085765No Hit
TTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTCT3850.29865334491746304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCG3730.28934466923172397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13800.042.87969645
ATGCCGT15700.037.69043444
CATCTCG16300.036.4410936
TATGCCG16300.036.4410943
TCTCGTA16300.036.4410938
CGTATGC16350.036.32964741
GTATGCC16350.036.32964742
TCGTATG16400.036.21888740
GCCCACG16550.036.16251818
AGCCCAC16550.036.16251817
CACGAGA16550.036.16251821
CCACGAG16550.036.16251820
CTCGTAT16450.036.108839
ACTCGTG16450.036.108827
CGTGTGC16400.036.08169630
CCCACGA16600.036.05359619
CGAGCCC16600.036.05359615
CGAGACT16600.036.05359623
ATCTCGT16500.035.9993837
ATCGTGT250.002100368535.999388
Produced by FastQC (version 0.11.2)