H23LNBCXX_l01n01_bir_0120_pe_24.35100000046a42.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_24.35100000046a42.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12987
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

$Duplication level graph$

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC	554	4.265804265804266	No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT	185	1.4245014245014245	RNA PCR Primer, Index 23 (95% over 21bp)
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	131	1.0087010087010087	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	101	0.7777007777007777	No Hit
ATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCTTC	92	0.7084007084007085	Illumina PCR Primer Index 2 (95% over 21bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87	0.6699006699006699	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70	0.539000539000539	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.4851004851004851	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.4543004543004543	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	58	0.44660044660044657	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	51	0.3927003927003927	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	43	0.3311003311003311	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.3234003234003234	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	0.3157003157003157	No Hit
TATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCTT	41	0.3157003157003157	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	0.3157003157003157	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.3003003003003003	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.3003003003003003	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	38	0.2926002926002926	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	37	0.2849002849002849	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	34	0.26180026180026184	No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.26180026180026184	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.24640024640024638	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	25	0.1925001925001925	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT	24	0.18480018480018479	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	24	0.18480018480018479	No Hit
GTACGGGAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	20	0.15400015400015402	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	18	0.1386001386001386	No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	14	0.10780010780010779	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	14	0.10780010780010779	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.10780010780010779	No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.10010010010010009	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	40	3.119203E-7	39.375	45
ATGCCGT	100	3.45608E-11	27.0	44
AGCCCAC	105	6.366463E-11	25.714285	17
GTATGCC	105	6.366463E-11	25.714285	42
ATCTCGT	105	6.366463E-11	25.714285	37
GCCCACG	105	6.366463E-11	25.714285	18
TGCCGTC	105	6.366463E-11	25.714285	45
AACTGGA	105	6.366463E-11	25.714285	31
CCACGAG	105	6.366463E-11	25.714285	20
CCTAACT	105	6.366463E-11	25.714285	28
TATGCCG	105	6.366463E-11	25.714285	43
GGATCTC	105	6.366463E-11	25.714285	35
CACGAGA	105	6.366463E-11	25.714285	21
CCCACGA	105	6.366463E-11	25.714285	19
TCTCGTA	105	6.366463E-11	25.714285	38
TAACTGG	105	6.366463E-11	25.714285	30
ACGAGAC	105	6.366463E-11	25.714285	22
CTAACTG	105	6.366463E-11	25.714285	29
CTGGATC	105	6.366463E-11	25.714285	33
GATCTCG	105	6.366463E-11	25.714285	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content