H23LNBCXX_l01n01_bir_0120_pe_22.351000000469f0.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_22.351000000469f0.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286155
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC	37829	13.219758522479076	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT	4912	1.7165522182034214	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG	1020	0.3564501756041306	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	942	0.3291922209991089	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.28725690622215233	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	619	0.21631633205780082	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	599	0.2093271129283081	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	555	0.19395083084342402	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	554	0.19360136988694937	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	499	0.17438101728084432	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	475	0.165993954325453	No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT	464	0.16214988380423195	RNA PCR Primer, Index 23 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	424	0.14817144554524647	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	388	0.1355908511121595	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	374	0.13069839772151456	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	364	0.1272037881567682	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT	351	0.12266079572259789	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	341	0.11916618615785152	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	341	0.11916618615785152	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	330	0.1153221156366305	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	317	0.11077912320246021	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	4115	43.682808	45
ATGCCGT	4620	38.95665	44
TATGCCG	4720	38.137962	43
CTCGTAT	4715	38.08296	39
CGTATGC	4725	38.049984	41
GTATGCC	4725	38.049984	42
GGATCTC	4685	38.03865	35
TCTCGTA	4720	37.947273	38
GATCTCG	4710	37.932297	36
TCGTATG	4740	37.929573	40
CTGGATC	4705	37.92478	33
ATCTCGT	4730	37.91462	37
ACTGGAT	4715	37.844345	32
TGGATCT	4715	37.790016	34
ACGAGAC	4750	37.75498	22
CCTAACT	4745	37.74734	28
CTAACTG	4740	37.73969	29
GAGACCT	4770	37.738194	24
CACGAGA	4755	37.71528	21
ACCTAAC	4755	37.71528	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content