FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_20.351000000469c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_20.351000000469c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374338
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC328428.773354561919975No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT69071.8451239254363705No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC21130.5644631322494643No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC16980.45360075653553744No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA14080.3761306626631547No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA13290.3550267405392987No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA12030.3213673204430221No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA11780.31468886407471325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA11670.3117503432726573No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC10700.285837932563619No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG9700.2591241070903835No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT9550.2551170332693982No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT8790.2348145259097393RNA PCR Primer, Index 23 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC8430.2251975487393746No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG7870.21023780647436274No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA7020.18753105482211263No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA6750.18031832194433906No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6590.1760441098686214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG6340.16936565350031255No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC6230.16642713269825665No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT6220.1661599944435243No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT5200.13891189246082417No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG5020.13410340387564182No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4920.13143202132831827No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4900.13089774481885355No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC4900.13089774481885355No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC4860.12982919179992414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG4540.1212807676484888No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4370.11673941731803877No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG4210.1124652052423211No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA4180.11166379047812405No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT4150.11086237571392699No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA4150.11086237571392699No Hit
GTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGCC4120.11006096094972992No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4080.10899240793080049No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC4010.10712244014767403No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3960.10578674887401225No Hit
GTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCC3950.10551961061927992No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC3950.10551961061927992No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA3930.1049853341098152No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3890.10391678109088577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.1031153663266887No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT3810.10177967505302696No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA3760.10044398377936517No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGAT207.027801E-444.99927
TGCCGTC37600.043.20401845
GGAAGCC301.138571E-437.49933219
ATGCCGT45750.035.50756544
TATGCCG47250.034.4279643
ATCTCGT47100.034.2987537
TCTCGTA47050.034.2873838
GATCTCG47000.034.27598636
CTCGTAT47200.034.27375439
CGTATGC47150.034.2623841
GTATGCC47300.034.24886342
GGATCTC47050.034.2395635
TCGTATG47250.034.1422540
CTGGATC47550.033.97415533
ACTGGAT47750.033.87897532
CCTAACT47950.033.87843728
ACGAGAC47950.033.87843722
TGGATCT47700.033.8673234
ACCTAAC47900.033.86683327
CACGAGA48000.033.84314721
Produced by FastQC (version 0.11.2)