FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_2.35100000046788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_2.35100000046788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10266
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTC255424.878238846678354No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGT2001.948178453146308No Hit
TTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCGTCT660.6428988895382818No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCTCGTATGCCG620.6039353204753555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2350.039.25531845
ATGCCGT2550.036.1764744
GCCGTCT250.002011381636.045
AGCCCAC2650.034.8113217
CCACGAG2650.034.8113220
GAGCCCA2650.034.8113216
CACGAGA2650.034.8113221
CCCACGA2650.034.8113219
ACGAGAC2650.034.8113222
CCGAGCC2650.034.8113214
GCTCTGG2650.034.8113230
TCCGAGC2650.034.8113213
GACAGCT2600.034.61538326
CGAGACA2600.034.61538323
GAGACAG2600.034.61538324
CTCTGGA2700.034.16666431
GTATGCC2700.034.16666442
ATCTCGT2700.034.16666437
GCCCACG2700.034.16666418
TCTGGAT2700.034.16666432
Produced by FastQC (version 0.11.2)