FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_19.351000000469a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_19.351000000469a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247716
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC2887611.656897414781444No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT91883.709086211629447No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC19420.7839622793844564No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC17280.6975730271762826No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA13510.5453826155759014No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA12690.5122801918325824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG11090.44769009672366744No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA9040.36493403736537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA8970.36210822070435494No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC8690.3508049540602949No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA8640.3487865135881413No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC8560.34555700883269547No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA7130.2878296113291027No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT7010.28298535419593407RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT6560.2648193899465517No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA5950.24019441618627785No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5470.22081738765360334No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG5360.2163768186148654No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC5150.20789936863182032No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT4890.1974034781766216No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC4890.1974034781766216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG4770.192559221043453No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC4740.19134815676016084No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4530.18287070677711573No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4040.1630899901500105No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3480.14048345686189023No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC3460.1396760806730288No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT3460.1396760806730288No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT3330.13442813544542945No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3290.13281338306770657No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA3260.1316023187844144No Hit
GTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGCC3240.13079494259555297No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3200.1291801902178301No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT3140.12675806165124578No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA3120.12595068546238433No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC3120.12595068546238433No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2990.120702740234785No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG2960.11949167595149285No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA2870.11585848310161637No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC2700.10899578549629416No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG2650.10697734502414055No Hit
GTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2650.10697734502414055No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG2630.10616996883527911No Hit
TATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG2620.10576628074084839No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA2600.10495890455198696No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA2580.10415152836312552No Hit
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC2540.10253677598540265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG2480.10011464741881831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31000.043.10826545
GTTAAGT250.002096706336.025213
GCACTCT250.00210503835.99612413
ATGCCGT40850.032.71373444
CGTCTTC352.8158125E-432.13939745
CACGAGA42050.031.94067621
GACCTAA42050.031.94067626
ACCTAAC42000.031.92513527
AACTGGA42000.031.92513531
CGAGACC42000.031.92513523
ACGAGAC42000.031.92513522
CTGGATC41600.031.90762333
CCTAACT42100.031.90274228
GAGACCT42100.031.90274224
AGACCTA42100.031.90274225
ACTGGAT41800.031.80877932
GGATCTC41350.031.77408835
GATCTCG41350.031.77408836
TAACTGG42200.031.77382930
CCACGAG42350.031.76753420
Produced by FastQC (version 0.11.2)