H23LNBCXX_l01n01_bir_0120_pe_18.35100000046986.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_18.35100000046986.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99483
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC	10980	11.037061608516026	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT	3092	3.1080687152578834	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	483	0.4855100871505685	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	470	0.47244252786908314	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	413	0.4151463064041092	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG	388	0.39001638470894523	No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT	382	0.3839852035021059	RNA PCR Primer, Index 23 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA	361	0.3628760692781681	No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC	359	0.360865675542555	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	295	0.2965330760029352	No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC	252	0.2533096106872531	No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG	251	0.2523044138194465	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	229	0.2301900827277022	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	217	0.21812772031402353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG	185	0.18596142054421358	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	158	0.15882110511343647	No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG	150	0.150779530170984	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG	149	0.1497743333031774	No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA	147	0.1477639395675643	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG	138	0.13871716775730528	No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC	133	0.13369118341827246	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT	131	0.13168078968265937	No Hit
GTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGCC	126	0.12665480534362655	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	122	0.1226340178724003	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.12162882100459375	No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC	118	0.11861323040117405	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGAG	118	0.11861323040117405	No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA	115	0.11559763979775439	No Hit
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG	113	0.11358724606214128	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	112	0.11258204919433472	No Hit
ATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCTTC	111	0.11157685232652814	Illumina PCR Primer Index 2 (95% over 21bp)
AAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA	110	0.11057165545872158	No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA	108	0.10856126172310847	No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC	106	0.10655086798749536	No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG	106	0.10655086798749536	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1175	0.0	42.698315	45
GCCGTCT	60	3.6379788E-12	41.246315	45
ATGCCGT	1530	0.0	32.791187	44
TATGCCG	1575	0.0	31.854296	43
CTCGTAT	1575	0.0	31.568607	39
GTATGCC	1590	0.0	31.553783	42
TAACTGG	1590	0.0	31.553783	30
CGTATGC	1590	0.0	31.553783	41
TCGTATG	1590	0.0	31.553783	40
GGATCTC	1555	0.0	31.540588	35
AGACCTA	1595	0.0	31.45487	25
TCTCGTA	1555	0.0	31.395908	38
GATCTCG	1555	0.0	31.395908	36
AACTGGA	1600	0.0	31.356571	31
CCTAACT	1600	0.0	31.356571	28
CGAGACC	1600	0.0	31.356571	23
CTGGATC	1565	0.0	31.339052	33
ACTGGAT	1580	0.0	31.326313	32
ACGAGAC	1605	0.0	31.258888	22
ACCTAAC	1605	0.0	31.258888	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content