FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_17.3510000004696c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_17.3510000004696c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73710
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC782310.61321394654728No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGT9561.2969746303079637No Hit
TTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCT2640.3581603581603582RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTCTTC2130.288970288970289Illumina PCR Primer Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCG2090.2835436168769502No Hit
TTTTCATGCTCTTCTTCATTGGAAGAGTGATGCATATCAACCTAGTTACCA980.13295346628679963No Hit
ATTCATAAGATCAATAACCTCTTTTCGAGGTGATGCATCTTCAAATAAGAC890.12074345407678741No Hit
GTTTTAGTTTTCAAACTTCTCAAAACAGAAAAGTTTTCTCTTGAACCTTGA880.11938678605345272No Hit
TGTCAGATAAATGCAAATGAAGTAACATTGAGATGCTACACCTTGTACACA850.11531678198344864No Hit
AATTAAGACTTCTGCTATTTGGAAAACACCATTAAAAGACAGAAATGCAAC830.11260344593677926No Hit
ATTCAACAATATGAGTTTAAAGCACACATCACAAAGAAGTCTCTCAGAATG810.10989010989010989No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGT253.8552625E-545.016
CGAAAGT206.990351E-445.014
AGGAGAG253.8552625E-545.015
CGTCTTC406.666596E-945.045
CAACACC206.990351E-445.010
TCGGAAA206.990351E-445.035
AGGTTAC206.990351E-445.037
TTCGGAA206.990351E-445.034
CCGAAAG206.990351E-445.013
CTCTCCG206.990351E-445.09
GGTTACA206.990351E-445.038
TGCCGTC8700.040.60344745
GAGAGTG301.12958856E-437.49999617
ATGCCGT9700.036.41752644
TGGAGGT250.002094366436.034
TACTTCC250.002094366436.026
TTTCGGA250.002094366436.033
TGTATTA250.002094366436.010
CCGTCTT504.7739377E-836.044
GAGGTTA250.002094366436.036
Produced by FastQC (version 0.11.2)