H23LNBCXX_l01n01_bir_0120_pe_15.35100000046929.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_15.35100000046929.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	204533
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC	9598	4.692641285269369	TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	2208	1.0795323981949123	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1773	0.8668527817027082	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	1496	0.7314223132697413	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1461	0.7143101602186445	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1401	0.6849750407024784	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1315	0.6429280360626403	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	1265	0.6184821031325018	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.4708286682344658	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	945	0.46202813237961604	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.4527386778661634	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	903	0.4414935487182997	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	825	0.40335789334728384	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	822	0.4018911373714755	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT	804	0.39309060151662567	TruSeq Adapter, Index 11 (95% over 23bp)
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	778	0.3803787163929537	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	608	0.2972625444304831	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	558	0.2728166115003447	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	556	0.2718387741831392	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	539	0.2635271569868921	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	525	0.25668229576645335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	471	0.23028068820190387	No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	406	0.1985009753927239	No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	380	0.18578909026905194	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	377	0.18432233429324363	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	364	0.17796639173140763	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	343	0.16769909990074952	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	303	0.1481423535566388	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	285	0.13934181770178894	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	273	0.13347479379855573	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	270	0.13200803782274742	No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	265	0.1295634445297336	No Hit
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT	256	0.1251631766023087	TruSeq Adapter, Index 11 (96% over 25bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	256	0.1251631766023087	No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.12369642062650037	No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	251	0.12271858330929483	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA	233	0.113918047454445	No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	231	0.11294021013723947	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	228	0.11147345416143116	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT	225	0.11000669818562285	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT	224	0.10951777952702009	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG	210	0.10267291830658133	RNA PCR Primer, Index 11 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	205	0.10022832501356749	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	1030	0.0	41.936504	45
TGGACGC	35	6.218019E-6	38.576042	30
CACGAGA	1155	0.0	37.796726	21
CCACGAG	1155	0.0	37.796726	20
AGACACG	1145	0.0	37.733772	25
TATGCCG	1145	0.0	37.724545	43
ATCTCGT	1125	0.0	37.5953	37
ACACGGC	1150	0.0	37.569706	27
GACACGG	1150	0.0	37.569706	26
GAGACAC	1150	0.0	37.569706	24
CGAGACA	1150	0.0	37.569706	23
ATGCCGT	1150	0.0	37.56052	44
TGCATCT	1130	0.0	37.438103	34
GTATGCC	1155	0.0	37.397926	42
CCCACGA	1170	0.0	37.312153	19
ACGAGAC	1170	0.0	37.312153	22
CATCTCG	1135	0.0	37.264065	36
ACGGCTG	1160	0.0	37.24583	29
CGTATGC	1160	0.0	37.236725	41
CTGCATC	1155	0.0	37.212242	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content