FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_0120_pe_13.351000000468d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_0120_pe_13.351000000468d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161437
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC103466.408691935553808TruSeq Adapter, Index 11 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT11970.7414657110823417TruSeq Adapter, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6520.4038727181501143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.35369834672348965No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5650.3499817266178138No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.2806048179785303No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.2806048179785303No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4380.27131326771434056No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4010.24839411039600587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.22609438976195048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.19760030228510192No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.19078649875802944No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3010.18645044196807423No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.18645044196807423No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3010.18645044196807423No Hit
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT2950.18273382186239834TruSeq Adapter, Index 11 (96% over 25bp)
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2790.17282283491392927No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG2740.1697256514925327RNA PCR Primer, Index 11 (95% over 22bp)
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.12760395696153917No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.12202902680302534No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.10716254638032173No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1680.10406536295892516No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1640.10158761622180788No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACT207.013586E-445.00216718
TGCCGTC10950.040.89238445
ATGCCGT12300.036.2212644
CTAACAA250.002098063536.01289434
GCAGCTA250.002101259336.00173645
AGCACTA250.002104458935.990587
TATGCCG12750.034.9428643
CCACGAG12850.034.8460420
GTATGCC12800.034.80636642
CGAGACA12800.034.63057323
TCTCGTA12750.034.57918538
ACACGGC12850.034.5065227
GACACGG12850.034.5065226
AGACACG12850.034.5065225
CGTATGC12850.034.49582741
ACGAGAC13050.034.4844222
CGGCTGC12800.034.46546630
GGCTGCA12800.034.46546631
CATCTCG12800.034.4441136
CCCACGA13000.034.4439719
Produced by FastQC (version 0.11.2)