H23LNBCXX_l01n01_bir_0120_pe_11.351000000468a1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_0120_pe_11.351000000468a1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119635
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTC	7802	6.521502904668367	TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	1056	0.8826848330338111	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	883	0.738078321561416	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	712	0.5951435616667363	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	689	0.5759184185230075	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.5692314122121453	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	671	0.5608726543235675	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGT	652	0.5449910143352698	TruSeq Adapter, Index 11 (95% over 23bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	626	0.5232582438249677	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	581	0.48564383332636774	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.4379989133614745	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	459	0.38366698708571906	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.3552472102645547	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	421	0.35190370710912355	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	405	0.33852969448739917	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	349	0.29172065031136374	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	329	0.2750031345342082	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	283	0.23655284824675052	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	261	0.21816358089187948	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	256	0.2139842019475906	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	256	0.2139842019475906	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	252	0.21064069879215946	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	202	0.1688469093492707	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	189	0.1579805240941196	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCG	188	0.15714464830526184	RNA PCR Primer, Index 11 (95% over 22bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	174	0.14544238726125297	No Hit
TTATACACATCTCCGAGCCCACGAGACACGGCTGCATCTCGTATGCCGTCT	162	0.13541187779495967	TruSeq Adapter, Index 11 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	156	0.13039662306181302	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.1270531199063819	No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA	150	0.12538136832866636	No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	148	0.1237096167509508	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT	140	0.1170226104400886	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	137	0.11451498307351528	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	134	0.11200735570694195	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	123	0.1028127220295064	No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	120	0.10030509466293308	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	20	7.007936E-4	45.000004	45
TGCCGTC	895	0.0	42.234634	45
ATGCCGT	965	0.0	39.170982	44
TATGCCG	995	0.0	37.989952	43
GTATGCC	990	0.0	37.954544	42
CGTATGC	995	0.0	37.76382	41
CACGGCT	995	0.0	37.76382	28
ACGGCTG	995	0.0	37.76382	29
CGAGACA	995	0.0	37.76382	23
CATCTCG	975	0.0	37.615383	36
GAGACAC	1005	0.0	37.61194	24
GACACGG	1000	0.0	37.574997	26
AGACACG	1000	0.0	37.574997	25
ATCTCGT	980	0.0	37.42347	37
TCTCGTA	980	0.0	37.42347	38
TCGTATG	980	0.0	37.42347	40
ACACGGC	1005	0.0	37.388058	27
CACGAGA	1005	0.0	37.388058	21
TGCATCT	990	0.0	37.272728	34
GCATCTC	990	0.0	37.272728	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content